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KMID : 1007519950040020093
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Food Science and Biotechnology
1995 Volume.4 No. 2 p.93 ~ p.97
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Quantification of Castor Bean Allergen in Castor Meal by Rocket Immunoelectrophoresis
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Kim Byongki
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Abstract
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A major castor bean allergen, CB-1A was isolated from commercially defatted castor meal. The isolated and purified antigenic fraction showed typical three major and two minor bands in disc gel electrophoretic pattern. CB-1A contained large amounts of glutamic acid and arginine followed by cysteine and serine. An antiserum (antibody) was produced by a subcutaneous injection of the purified CB-lA into rabbits for the in vitro antigen-antibody precipitin reaction. A Rocket immuno-electrophoresis was employed to quantitatively analyse the amount of antigenic fraction in the defatted castor meal. A strong linear correlation existed between the peak height of the electrophoresed precipitin lines and the various concentrations of a purified CB-1A solution as a standard. The CB-1A content of the commercial castor meal purchased for this experiment was estimated approximately as 0.49% (w/w).
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